Journal: Scientific Reports

Article Title: Enhanced axonal regeneration of ALS patient iPSC-derived motor neurons harboring SOD1 A4V mutation

doi: 10.1038/s41598-023-31720-7

Figure Lengend Snippet: Antibodies/Reagent used for immunofluorescence staining.

Article Snippet: Stathmin-2 (STMN2) , Early regeneration marker , Novus , 1:2000.

Techniques: Immunofluorescence, Staining, Marker

Journal: Scientific Reports

Article Title: Enhanced axonal regeneration of ALS patient iPSC-derived motor neurons harboring SOD1 A4V mutation

doi: 10.1038/s41598-023-31720-7

Figure Lengend Snippet: SOD1 +/+ and SOD1 +/A4V axons regenerate similarly 24 h following axotomy. ( A ) Mean axon length 24 h post-axotomy (SOD1 +/+ n = 13; SOD1 +/A4V n = 12). Each data point represents 50–100 traced axons from 1 individual axonal compartment. ( B ) Frequency distribution of axon length 24 h post-axotomy. ( C ) SOD1 +/+ (left) and SOD1 +/A4V (right) regenerating axons 24 h post axotomy stained for GAP-43. Scale bar = 50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu$$\end{document} μ m. ( D-E ) Growth cones 24 h post axotomy stained for smi-31 (green), F-actin (white), stathmin-2 (red). Scale bar = 2 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu$$\end{document} μ m. ( D ) SOD1 +/+ (top panels) ( E ) SOD1 +/A4V (bottom panels). ( F ) Average growth cone area 24 h post-axotomy. (G) Average STMN2 fluorescence intensity in growth cone area 24 h post-axotomy. Bars represent mean ± SEM. ns indicates p > 0.05.

Article Snippet: Stathmin-2 (STMN2) , Early regeneration marker , Novus , 1:2000.

Techniques: Staining, Fluorescence